siRNA Libraries

The Yale Center for HTCB offers access to Dharmacon's human siGenome library. We are looking to expand our capabilities to better address the needs of the Yale scientific community and welcome any feedback or suggestions. Future library purchases will be made based on interest in projects in specific areas.

The siGenome collection is a human full genome library consisting of roughly 18,119 genes arrayed in 58 384-well plates with columns 1, 2, 23 and 24 empty and thus eligible for control testing. Each sample well contains a pool of 4 different siRNA targeting the same gene. Clients can choose to screen the full genome library or the following subsets of the library:

The full genome library is made up of the following subsets:

Druggable Genome (9 plates - 2,739 genes)Download gene list

(ion channels, proteases, phosphatases, kinases, ubiquitin conjugation proteins, and G-protein coupled receptors)

Drug Targets (15 plates - 4,795 genes)Download gene list

(genes involved in apoptosis, senescence, nucleic acid binding, autophagy, DNA repair, and characterized nuclear receptors, potential therapeutic targets)

Human Genome (34 plates - 10,582 genes)Download gene list

(genes not contained in other subsets including uncharacterized genes)

Smaller subsets also available for screening are:

Kinases (3 plates - 714 genes)Download gene list

(genes with known or predicted kinase activity)

Phosphatases (1 plates - 256 genes)Download gene list

(genes with known or predicted phosphatase activity)

In addition to the pre-arrayed subsets of the library above, custom subsets can be acquired pooled or unpooled, in various plate formats (contact HTCB staff for further information).